This study's intended purpose was to understand the effectiveness of dietary fiber treatments and stimbiotic on the sustainability of the F18 enterotoxigenic Escherichia coli (ETEC) challenge. A total of 216 piglets were blocked by weaning BW and randomly assigned to 1 of 4 dietary treatments, including corn-SBM based control diet (CC), high fiber diet (HF: 5.0, 3.8. 2.5% sugar beet pulp and 4.0, 3.0 and 2.0% soy hulls for phases 1, 2, and 3, respectively), CC plus 0.01% stimbiotic (CS: Signis, ABVISTA Marlborough, United Kingdom), and HF plus 0.01% stimbiotic (FS). Pigs were fed a four-phase feeding regimen: phase 1 (d0-9), phase 2 (d9-16), phase 3 (d16-22), phase 4 (d22-41). Dietary fiber and stimbiotic were provided from weaning (d0) to d22, and then a common diet was fed from d22 to the end of the trial. On d5 postweaning, all pigs were orally inoculated with 10 ml of F18 ETEC (10⁸ CFU/ml.) One median BW pig from each pen was selected at weaning for fecal sampling on d8. Samples were used to quantify the live E. coli using 3M Petrifilm (3M Food Safety, St. Paul, MN) and virulence genes of the isolate were qualified using multiplex PCR. Of 466 colonies, 53 colonies that expressed F18 also presented LTb, Stb, and Stap simultaneously. Pigs fed high fiber diets (HF and FS) decreased the percentage of F18 encoded E. coli ( P < 0.0001) than low fiber diets (CC and CS), while pigs fed stimbiotic (CS and FS) had higher percentage of F18 encoded E. coli (P < 0.0001) than diets devoid of Signis (CC and HF). Results of this study demonstrated that high fiber and stimbiotic had a distinguished result on the percentage of virulence genes expressed in live E. coli colonies thus suggesting a different mechanism to mitigate ETEC challenge.