Chagas’ disease is an insect-vectored disease caused by the zoonotic protozoan parasite, Trypanasoma cruzi. Chagas’ disease can be fatal in both humans and animals. Trypanosoma cruzi is endemic in many wildlife species in the United States, but little is known about T. cruzi infections in bears. Only 3 cases of T. cruzi-infected bears have been reported worldwide. This study was conducted to determine the prevalence of antibodies to T. cruzi in black bears from the United States. Sera from 50 black bears originating in Pennsylvania were tested for antibodies to T. cruzi with rapid immunochromatographic dipstick assays. Tests are being conducted currently, but at present, anti-T. cruzi antibodies were detected in 1 of 27 (3.7%) samples tested so far. These results suggest that black bears are exposed to T. cruzi at a low level in Pennsylvania. Antibody test results from all 50 bears will be presented.