The gender binary that most people are familiar with hasn’t always been the societal focus that it is today. Many artists throughout history have portrayed individuals in their artworks as gender fluid and non-binary. Some examples include Bernini’s Sleeping Hermaphroditus and Caravaggio’s Supper at Emmaus. I will be focusing on how gender fluidity was expressed in Baroque art. Artists uniquely experimented with gender identities and forms of expression. I want to determine if they had any reason for this or if they saw the gender binary as a hindrance in human expression. Non-binary expression and gender fluidity is something that isn’t new. It has been around for centuries. Many cultures have their own expressions of gender fluidity. Many people oppose the rejection of the binary and are convinced that there can only be males or females, which is not true. I will examine how non-binary and gender fluid individuals were shown during the Baroque period. My research is intriguing because I want to tear down the beliefs that an individual can only be a “male” or “female.” The gender binary was established under colonialism. Many cultures had been fluid with gender until colonialism. With this topic I hope to give people more knowledge on genderqueer, non-binary, and gender fluid individuals. I will gather evidence through books on artists such Caravaggio and Bernini. I will compare the works of these famous Italian Baroque artists with paintings of other artists during the 17th century, researched through monographs, art history texts and scholarly articles. I will examine these artworks and try to decipher what they are trying to say, in terms of gender identity. I will also contextualize the artworks with my research on the notion of gender identity in Italy in the 17th century.