Neurons are formed and integrated into neuronal circuits during embryonic development. After birth, they undergo functional and anatomical changes driven by tightly regulated genetic programs. The underlying mechanisms contributing to neuronal maturation and how these neurons mature post-birth are poorly understood. Alternative splicing (AS) is a post/co-transcriptional mechanism in which splicing factors (SFs) are recruited and act upon premature mRNA strands to produce multiple mRNA isoforms from one gene. The expression of these isoforms can change depending on the stage of development, causing differences in how an individual gene is expressed. This isoform-switching process allows for greater functional diversity within a specific gene. This study aims to test the hypothesis that AS regulation alters neuronal isoform expression, contributing to neuronal maturation. I will use the model Caenorhabditis elegans due to its well-characterized cell lineage, rapid development cycle, and ease of genetic amenability. I will focus on the exc-7 and unc-75 SFs (mammalian orthologs are ELAVL and CLEF families). Both contribute heavily to neurological processes and maturation, such as aiding in the regulation of neuron differentiation and synaptic transmission. Computational methods allow for the analysis of bulk and single-nuclei RNA-sequencing data generated in the lab and published for public access across developmental stages in C. elegans. Utilizing developmental bulk RNA-sequencing data across the entire nervous system (NS) of C. elegans, I can evaluate NS-wide SF expression changes across different larval stages. With this, I will be able to identify isoform switching that contributes to expression changes found in the differential expression analysis. Knowing that the NS comprises multiple neuronal cell types, I will be using single-cell methodologies to evaluate SF expression changes in specific cell types. Completing this, I expect to evaluate exc-7 and unc-75 SF expression changes across whole NS development and specific neuronal cell-types.