The purpose of this investigation was to find the environmetal factors affecting E.coil levels in the Kinnickinnic River at three sample sites in Milwaukee: Zablocki Park, Wilson Park, and Jackson Park. This research holds significant value for the community, as the data collected is shared with Milwaukee Riverkeeper, a non-profit oganization dedicated to monitioring the river's overall health. E.coli, a pathogenic bacterium found in waterways, can cause illness in humans, making it crucial to montior its levels to ensure public safety and health. During the summer, water samples were collected four times from each of the three sites along the Kinnickinnic River. These samples underwent a series of tests, including presumptive test, confirmed test and completed test. The presumptive test is when the water sample is aseptically added to lactose broths; tubes were incubated for 24 hours at 37 C. Durham tubes with a gas bubble of 5mm or greater were marked as positive. Second, the confirmed test is where an Eosin-Methylene Blue (EMB) agar plate was inoculated for every positive lactose tube and incubated at 37 C for 24 hours. After 24 hours, all plates with metallic green were recorded as positive for E.coli. Last, the completed test is where all EMB plates that were positive for E.coli were used to inoculate an agar slant. The slants are gram-stained and miscroscopy is used to find the presence of E.coli. The results indicated that E.coli levels were impacted by water and air temperture, water velocity, pH levels, trash and wildlife.