E-cigarettes are often marketed as safer alternatives to cigarettes. It is a common misconception that e-cigarette vapor is harmless and consists only of flavored water. A study previously published by Harvard suggests that over 90% of e-cigarettes contain 2,3-butanedione, also known as diacetyl, a food flavoring known for its intense buttery flavor.  It is often substituted with 2,3-pentanedione. Both flavorings have been linked to severe respiratory disease and damage. In 2019, both were proposed as additions to the U.S. Food and Drug Administration’s “Harmful and Potentially Harmful Constituents List”, but they currently remain “generally recognized as safe” by the FDA. The goal of this study was to develop an effective method to analyze the 2,3-butanedione and 2,3-pentanedione levels in e-cigarette juice using headspace gas chromatography (HS-GC). HS-GC was used because of its effectiveness in detecting volatile organic compounds. This method allows for analysis of 2,3-butanedione and 2,3-pentanedione in the vapor phase, which minimizes sample preparation. Of the eight e-cigarette samples tested in this study, 75% contained detectable levels of diacetyl and 100% contained detectable levels of pentanedione. Given the increase in vaping in the United States, especially in younger populations, this data shows a need for further research and regulation of e-cigarettes.