Bacteriophages (phages) are a type of virus that infects bacteria. Like human viruses, phages use their host cells for replication and reassembly. Phages are present where there is bacterial contamination, so one can determine and quantify bacterial presence by analyzing environments for phages, whether in water or soil. Harmful bacteria in water and soil can be present because of fecal contamination from animals in the region. These fecal-borne bacteria can be pathogenic to humans, like E. coli. The natural replication of phages allows testing to determine the presence of these fecal-borne bacteria in bodies of water. Previous investigations into finding and extracting bacteriophage from Zumbro River and Silver Lake have shown inconclusive results, producing uncertainty in whether we have bacteriophage in our waterways. Zumbro River and Silver Lake were chosen due to signs of fecal contamination populating the water with bacteria that serve as a host cell for bacteriophage replication. Chloroform was used to isolate the phage from water and soil samples from both locations. To ensure we only have bacteriophage, a filter allowed the phage to travel to a sterile microcentrifuge tube before inoculating it with an E.coli broth. Our results showed positive for bacteriophage within soil surrounding fecal-contaminated waterways using Silver Lake samples. Zumbro River samples produced negative results for bacteriophage prevalence. Due to the widespread presence of fecal contamination in Rochester waterways, further analysis of bacteriophage presence using these methods is warranted to further understand our overall water quality.