PARE is the prevalence of antibiotic research in the environment and it is the basis of this research project. Antibiotic resistance is prevalent when trying to treat diseases, but it is also present in the soil and other aspects of the environment. The presence of resistant bacteria is an issue because it can build from lower trophic levels and harm humans and animals. Most of the antibiotic resistance comes from human or animal waste or the runoff water from agricultural land. To combat antibiotic resistance in the environment, the prominent geographic regions of resistance must first be located. Several institutions across thirty of the United States and Puerto Rico, including Tufts School of Medicine, are investigating antibiotic resistance in the environment through the PARE project1. The IU Indianapolis chapter of PARE has the following goals: determine the prevalence of antibiotic-resistant bacteria across Central Indiana and to examine the biological factors that antibiotic resistance is affected by. To achieve these goals, soil samples from different locations across Central Indiana were plated in a serial dilution on plates with varying concentrations of Tetracycline to test the antibiotic resistance. The biological factors that antibiotic resistance is affected by were tested by growing the plates at varying temperatures and pH concentrations. Keywords: Antibiotic resistance, PARE project, soil sampling, Temperature, pH